Antisense oligonucleotides against inducible nitric oxide synthase reduce No production in RAW 264.7 cells
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Several oligodeoxyribonucleotides in a nuclease-resistant phosphorothioate form targeted to iNOS mRNA were tested in RAW 264,7 cells as potential antisense inhibitors.Antisense S-ODNs inhibited iNOS activity in a time- and dose-depended manner.Application of Lipofectin agent, which itself had no significant effect, greatly increased antisense activity.Decreased levels of the target mRNA, as demonstrated by reverse transcriptase -polymerase chain reaction (RT-PCR), suggest tha RNase H mediated mechanism.
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R.Przewlocki, Institute of Pharmacology, Polish Academy of Sciences, Smetna St. 3, 31-343 Krakow, Poland,