Study on an embryo culture of black currant hybrids obtained as a result of distant hybridisation
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The subjects of this study were embryos obtained from 22 crossing combinations in the course of distant hybridisation. No embryos were obtained from the following three interspecific hybridisations: Siewka K1 x Josta, Siewka K1 x Czereszniewa and Captivator x Josta. Seeds obtained from these combinations did not hold endosperm, but yielded watered liquid. In the remaining hybridisations, a high percentages of visible embryos increased considerably, especially at the torpedo-shaped stage. An addition of kinetin (1 mg/l) to the media had a better impact on plant regeneration from embryos than the addition of BA (0,5 mg/l). Dependending on the study, up to 83,7% of exposed embryos in the media started to grow and develop under the influence of kinetin.
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A. Golis, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland, e-mail: firstname.lastname@example.org