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Abstracts
Reverse- transcription polymerase chain reaction has been applied to the detection of FMD viral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a template for the amplification by RT-PCR of the 424 bp of the VP1 coding sequence. Random primers p(dN)6 gave better results.
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114-120
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author
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article
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G. Paprocka, Zaklad Pryszczycy, Panstwowy Instytut Weterynaryjny, ul. Wodna 7, 98-220 Zdunska Wola, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-ae15a1d5-143a-3bc9-b37e-cf6584ad25e2