Efficiency of detecting RNA of FMDV using PCR method according to the starters used for cDNA synthesis

• Authors: G. Paprocka
• Languages of publication: PL

Abstracts

EN

Reverse- transcription polymerase chain reaction has been applied to the detection of FMD viral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a template for the amplification by RT-PCR of the 424 bp of the VP1 coding sequence. Random primers p(dN)6 gave better results.

Keywords

EN

FOOT-AND-MOUTH DISEASE   PRIMER  

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1998
• Issue: 3
• Pages: 114-120
• Document Type: article
• Publication order reference: G. Paprocka, Zaklad Pryszczycy, Panstwowy Instytut Weterynaryjny, ul. Wodna 7, 98-220 Zdunska Wola, Poland