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Journal
1998 | 3 | 114-120
Article title

Efficiency of detecting RNA of FMDV using PCR method according to the starters used for cDNA synthesis

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Languages of publication
PL
Abstracts
EN
Reverse- transcription polymerase chain reaction has been applied to the detection of FMD viral in samples. Total RNA was extracted with quanidinum thiocyanate-phenol-chloroform method and reversely transcribed using specific or random primers. cDNA was used as a template for the amplification by RT-PCR of the 424 bp of the VP1 coding sequence. Random primers p(dN)6 gave better results.
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Journal
Year
Issue
3
Pages
114-120
Physical description
Contributors
author
References
Document Type
article
Publication order reference
G. Paprocka, Zaklad Pryszczycy, Panstwowy Instytut Weterynaryjny, ul. Wodna 7, 98-220 Zdunska Wola, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-ae15a1d5-143a-3bc9-b37e-cf6584ad25e2
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