Regeneration of Arabidopsis thaliana (L.) Heynh. plants via direct somatic embryogenesis
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In Arabidopsis biotechnology plants are regenerated in vitro via shoot organogenesis induced in callus derived from different somatic tissues. An alternative way of in vitro plant regeneration via somatic embryogenesis has not been applied in Arabidopsis so far. Recently, it was found that development of Arabidopsis somatic embryos can be induced in the culture of immature zygotic embryos and that the callus phase is not nocessary for the initiation of embryogenesis. The aim of the presented research was to determinate the in vitro culture conditions enabling high efficiency of somatic embryo induction and their conversion into plants. The influence of induction medium composition including liquid or agar medium, type and concentration of auxin, carbohydrates and ammonium sources as well as duration of auxin treatment of explants on DSE efficiency were evaluated. Advantages of described regeneration system via DSE are as follows: short time needed to induce somatic embryos (10-15 days), high efficiency of the process (up to 90% explants responded), numerous embryos produced per explant (on average 17) and high percentage of embryo conversion into fertile plants (70-80%).
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M.D. Gaj, Katedra Genetyki, Uniwersytet Slaski, ul. Jagiellonska 28, 40-032 Katowice, Poland, e-mail: email@example.com