A new diagnostic SSR marker for selection of the Rym4/Rym5 locus in barley breeding

• Authors: M. Tyrka , D. Perovic , A. Wardynska , F. Ordon
• Languages of publication: EN

Abstracts

EN

Genomic sequence AY661558, representing a part of the BAC contig of the Rym4/Rym5 locus conferring resistance to the barley yellow mosaic virus complex (BaMMV/BaYMV), was exploited in order to develop SSR markers for practical barley breeding. Out of 57 SSR motifs found within this sequence, primers were designed and tested for the 5 SSRs with the highest repeat length. The polymorphic SSR marker QLB1 co-segregated with rym4 and rym5 phenotypes in respective high-resolution mapping populations developed for the construction of the original BAC contig. The primers targeted 2 sites located 756 bp and 5173 bp downstream of the translation initiation factor 4E (Hv-eIF4E). Physical linkage of the QLB1 marker to the Rym4/Rym5 locus was confirmed experimentally on Morex BAC 519J14, a seed BAC of Hv-eIF4E, and BAC 801A11, which is located proximally to Hv-eIF4E. QLB1 revealed 7 alleles in a set of 100 winter barley lines and cultivars. Five alleles were found within 673 advanced breeding lines derived from applied Polish winter barley breeding programmes, which corresponds to a PIC value of 0.684. No recombinants between Rym4/5 and QLB1 were detected, suggesting that QLB1 can be used efficiently in marker-assisted selection of the Hv-eIF4E-mediated bymovirus resistance.

Keywords

EN

HORDEUM VULGARE; MARKER; RYM4/RYM5 LOCUS; SSRS MARKER; WINTER BARLEY; YELLOW MOSAIC VIRUS

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2008
• Volume: 49
• Issue: 2
• Pages: 127-134

Contributors

author

M. Tyrka

author

D. Perovic

author

A. Wardynska

author

F. Ordon

• Document Type: ARTICLE
• Publication order reference: Dragan Perovic, Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany