Morphometric analysis of LPS-stimulated human monocytes by computer-assisted image analysis

• Authors: Z. Madeja , M. Kupiec , W. Korohoda
• Languages of publication: EN

Abstracts

EN

This report describes the results of applying the computer-assisted image analysis system for the measurement of some cytological parameters of LPS-stimulated and nonstimulated human monocytes. The experiments were carried out by means of the digital cell image analysis of haematoxilyn stained monocytes. Five different parameters describing the morphology of monocytes and their nuclei were selected to quantitate the differences between control and activated cells: area, perimeter, elongation, dispersion, and extension of images of cell projections. The results suggest that all of the analysed parameters can be used to discriminate stimulated from nonstimulated monocytes which permits detailed monitoring of the changes in cell morphology during monocyte activation.

Keywords

EN

CELL IMAGE ANALYSIS; MONOCYTE; SHAPE PARAMETER

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 1998
• Volume: 46
• Pages: 123-128

Contributors

author

Z. Madeja

author

M. Kupiec

author

W. Korohoda

• Publication order reference: Z. Madeja, Department of Cell Biology, The Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Mickiewicza 3, 31-120 Krakow, Poland. E-mail: [e-mail].p