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Journal
2004 | 2 | 123-130
Article title

Screening of Polish red clover cultivars for their capability to somatic embryogenesis

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Languages of publication
PL
Abstracts
EN
Ten Polish red clover cultivars were tested in vitro for their capability to produce callus and somatic embryos. A three-step tissue culture protocol (callus induction, embryo induction and plant development) based on Gamborg's B5 basal salts, NAA and 2,4-D as auxins, and kinetin and adenine as cytokinins was utilized. Explants source were petiole and hypocotyl sections. All clover varieties under study have showed high efficiency of callus production, and low ability for plant regeneration from the callus. The highest percent of genotypes which developed embryos was observed on variety Parka (3,6). Single embryogenic genotypes were selected from nearly all varieties. Hypocotyls showed the greatest ability for callus regeneration comparing to leaf petioles.
Publisher

Journal
Year
Issue
2
Pages
123-130
Physical description
Contributors
author
References
Document Type
ARTICLE
Publication order reference
R. Dolinski, Instytut Genetyki i Hodowli Roslin, Akademia Rolnicza, ul. Akademicka 15, 20-934 Lublin, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-a48cd03b-c949-35fb-8d04-795d2f05d413
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