In vitro embryo production in cattle ? technology and practical application
Languages of publication
Technology of bovine in vitro embryo production (IVP) had developed during the last few years to the level which allows its practical applications. Recently, IVP technology has been commercially used in several countries to increase the number of calves originating from donors of high genetic value. Combined with ovum pick-up technique (OPU), IVP technology serves as en alternative or integral method in breeding program MOET. The technology of IVP includes three developmental steps, i.e. oocyte in vitro maturation, in vitro fertilization and embryo culture. This review will focus on immature bovine oocyte recovery and selection, in vitro maturation, sperm processing, embryo culture to hatched blastocysts stage and factors affecting IVF efficiency. Moreover, recently developed investigations (IVP using calf oocytes and oocytes originating from small ovarian follicles) aiming to increase the effectiveness of IVP technology are discussed as well.
Publication order reference
L. Katska-Ksiazkiewicz, Zaklad Fizjologii Rozrodu Zwierzat, Instytut Zootechniki, 32-083 Balice k. Krakowa, Poland