EN
In situ hybridization (ISH) provides a highly sensitive method for the detection of specific nucleic acid sequances in cells, nuclei and metaphase chromosomes.This technique has been improved continuously since it was first established in 1969.Especially fluorescent in situ hybridization (FISH) rapidly replaced radioactive procedures and became a conventional tool in cytogenetic research.ISH plays an increasingly important role in a variety of reserch areas of medicine, genetics and plant breding.It has been succesfully applied for chromosome or chromosome fragments identification, chromosomal abormlities detection, gene mapping or specific DNA sequences localization.