Nucleic acids in situ hybridization: from ISH to DIRVISH

• Authors: J. Maluszynska
• Languages of publication: PL

Abstracts

EN

In situ hybridization (ISH) provides a highly sensitive method for the detection of specific nucleic acid sequances in cells, nuclei and metaphase chromosomes.This technique has been improved continuously since it was first established in 1969.Especially fluorescent in situ hybridization (FISH) rapidly replaced radioactive procedures and became a conventional tool in cytogenetic research.ISH plays an increasingly important role in a variety of reserch areas of medicine, genetics and plant breding.It has been succesfully applied for chromosome or chromosome fragments identification, chromosomal abormlities detection, gene mapping or specific DNA sequences localization.

Keywords

EN

CHROMOSOME; FLUORESCENT; GENE MAPPING; IN SITU HYBRIDIZATION; MOLECULAR CYTOGENETICS

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 1995
• Issue: 2
• Pages: 92-101

Contributors

author

J. Maluszynska

• Document Type: article
• Publication order reference: J.Maluszynska