Classic techniques for improvement of industrial yeast strains. Part II ? Transmission of killer activity into laboratory and industrial strains of Saccharomyces cerevisiae by electrotransformation

• Authors: A.T. Salek
• Languages of publication: EN

Abstracts

EN

Killer-sensitive strains of Saccharamocyces cerevisiae and Saccharomyces uvarum var. carlsbergensis were transformed by electroinjection using dsRNA isolated from a superkiller strain. Various recipient strains were used: both thermo-resistant and thermo-sensitive as well as mutants of industrial strains. Conversion of respiratory competent (rho+) into respiratory deficient (rho-) strains (mutants) resulted in a significant increase in the yield of electrotransformants and/or of long-term killer stability. Electrotransformation of rho- mutants of distillery and brewery strains resulted in more than 100 clones, which exhibited weak or strong killer activity over some or all of the experimental period of 10 months.

Keywords

EN

ELECTROTRANSFORMATION; INDUSTRIAL STRAIN; SACCHAROMYCES CEREVISIAE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Journal: Biotechnologia
• Year: 2002
• Issue: 1
• Pages: 175-186

Contributors

author

A.T. Salek

• Document Type: ARTICLE
• Publication order reference: A.T. Salek, Milan-Science, Institut of Microbiology, Dr. Ernst-Derra Str. 4, 94036 Passau, Germany