PL EN


Preferences help
enabled [disable] Abstract
Number of results
2008 | 56 | 2 | 127-134
Article title

1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophages

Title variants
Languages of publication
EN
Abstracts
EN
1-Methylnicotinamide (MNA), a major metabolite of nicotinamide (NA), is known to exert anti-inflammatory effects in vivo. Treatment of inflammatory skin diseases by topical application of MNA provides certain advantages over the use of NA. However, in contrast to NA, the molecular mechanisms of the anti-inflammatory properties of MNA are not well known. In this study the influence of exogenous MNA and NA in vitro on the generation of inflammatory mediators by macrophages (Mo) was investigated. Materials and Methods: Peritoneal Mo of CBA/J mice were activated in vitro with lipopolysaccharide and incubated with MNA or NA. The effect of these compounds on biological functions of Mo was measured by evaluation of the production of reactive oxygen species (ROS) by luminol-dependent chemiluminescence, cytokines and prostaglandin E2 (PGE2) by ELISA, and nitric oxide (NO) by the Griess method. Moreover, the expressions of inducible NO synthase and cyclooxygenase-2 were measured by Western blotting. Results: It was shown that at non-cytotoxic concentrations, NA inhibits the production of a variety of pro-inflammatory agents, such as tumor necrosis factor alpha interleukin 6, NO, PGE2, and the generation of ROS. In contrast to NA, exogenous MNA inhibited only the generation of ROS, while its effect on the synthesis of other mediators was negligible. Conclusions: These results indicate that the anti-inflammatory properties of MNA demonstrated previously in vivo do not depend on its capacity to suppress the functions of immune cells, but more likely may be related to its action on vascular endothelium. The authors suggest that the limited permeability for exogenous MNA, in contrast to that for NA, may be responsible for its lack of suppressor activity against Mo.
Publisher

Year
Volume
56
Issue
2
Pages
127-134
Physical description
Contributors
author
author
author
author
author
References
Document Type
ARTICLE
Publication order reference
Janusz Marcinkiewicz, Department of Immunology, Jagiellonian University Medical College, Czysta 18, 31-121 Krakow, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-9e7776a7-c584-312d-b257-529560d42e83
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.