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Journal
2010 | 4 | 76-90
Article title

Sequencing of the largest chromosome of Paramecium tetraurelia

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Languages of publication
EN
Abstracts
EN
The largest, megabase, somatic chromosome of P. tetraurelia was isolated and sequenced in order to explore its organization and gene content. The AT-rich chromosome is compact with very small introns, short intergenic regions and a coding density of at least 74%, higher than that reported for budding yeast or any other free-living eukaryote. Homology to known proteins could be detected only for 57% of the 464 potential protein coding genes. Subsequently, the megabase chromosome sequence was used during the whole genome sequencing project as a reference to evaluate sequence assembly and gene annotation accuracy. In a pilot project of the global analysis of P. tetraurelia gene expression during autogamy, DNA microarrays were used. Statistical data analysis allowed the identification of four clusters of co-expressed genes. Screening for silencing phenotypes of 15 autogamy specific genes revealed that 4 genes were essential during vegetative growth and 3 others were essential for successful sexual process.
Publisher

Journal
Year
Issue
4
Pages
76-90
Physical description
Contributors
author
References
Document Type
REVIEW
Publication order reference
Jacek K. Nowak, Instytut Biochemii i Biofizyki, Polska Akademia Nauk, ul. Pawinskiego 5A, 02-106 Warszawa, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-9ac7f2c2-2e6f-3aca-9281-9481e4e10bc5
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