Proteolysis of endomorphin-1 by brain synaptic membrane-associated peptidases

• Authors: L. Wu , J. Chen , D. Zhong , C. Lin , X. Wang
• Languages of publication: EN

Abstracts

EN

The biotransformation of endomorphin-1 (EM-1) by brain synaptic membranes has been studied. Peptide fragments of EM-1 that were formed during incubation with membrane preparations in vitro were isolated by High-performance liquid chromatography (HPLC) and characterized using determination of amino acid composition. At pH 7.4, two degradation products, EM-1(1-3) and EM-1(1-2), were identified. EM-1 was degraded 77.5% at 30 min incubation with synaptic membranes. The time course of the experiments and the effect of carboxypeptidase inhibitor (CPI) demonstrated that the proteolysis reaction involves the participation of carboxypeptidase activity.

Keywords

EN

BRAIN; DEGRADATION; ENDOMORPHIN; PROTEOLYSIS; SYNAPTIC MEMBRANE

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Journal: Acta Neurobiologiae Experimentalis
• Year: 2002
• Volume: 62
• Issue: 1
• Pages: 1-5

Contributors

author

L. Wu

author

J. Chen

author

D. Zhong

author

C. Lin

author

X. Wang

• Document Type: ARTICLE
• Publication order reference: J. Chen, Pharmaceutic Biotechnology Key Laboratory, Department of Biochemistry, Nanjing University, Nanjing 210093, P. R. China