Microspores cultured in vitro can be reprogrammed to divide and produce a bipolar embryo. The reaction to stress treatment is a signal for inducing the sporophytic pathway, preventing the development of fertile pollen grain ? the gametophytic pathway. The ultimate goal is to convert each microspore from a heterozygous F1 plant to a doubled haploid plant so that a population of doubled haploids fully represents the genetic variability of the preceding meiosis.
T. Cegielska-Taras, Pracownia Kultur Tkankowych, Zaklad Roslin Oleistych, Instytut Hodowli i Aklimatyzacji Roslin, ul. Strzeszynska 36, 60-479 Poznan, Poland