Epithelial cell cultures as a model system to study the transepithelial transport

• Authors: W. Grajek , A. Olejnik , K. Staniaszek
• Languages of publication: PL

Abstracts

EN

Epithelial cell lines are commonly accepted model system to determine intestinal permeability and transepithelial transport. The application of Caco-2 human epithelial cell culture can be used to study bioavailability of drug and food compounds. This culture grows on porous membranes and forms a monolayer of well differentiated cells joined by intracellular tight junctions. This system provides selective barrier to study passive and active transport of different molecules. The Caco-2 cells posses some enzymes and transport systems similar to intestinal epithelium in vivo, however, these proteins are expressed to a lesser extent compared to normal enterocytes. In this paper, the advantages and limitations of Caco-2 model are discussed.

Keywords

EN

BIOAVAILABILITY; EPITHELIUM; TRANSPORT

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2006
• Issue: 2
• Pages: 148-165

Contributors

author

W. Grajek

author

A. Olejnik

author

K. Staniaszek

• Document Type: REVIEW
• Publication order reference: Wlodzimierz Grajek, Katedra Biotechnologii i Mikrobiologii Zywnosci, Akademia Rolnicza im. Augusta Cieszkowskiego, ul. Wojska Polskiego 48, 60-627 Poznan, Poland