Experiments were carried out on <Lupinus albus> (cvs. Wat and Hetman).L.luteus (cvs. Topaz and Iryd), <L. angustifolius> (cv, Remik), <L.hispanicus> and <L.polyphyllus>. For culture initiation 5- and 14-day-old seedling explants were used. The <rooting process> of the <shoot tip> was stimulated by various media but the universal one appeared to be Gamborg's B5 medium solidified with Gelrite. For each investigated species a medium assuring 100% rooting of explants was developed. The most effective combination of growth substances for multiplication of L. hispanicus was found. Cotyledonary node explants, cultured on medium supplemented with benzyladenine and with or without naphthaleneacetic acid were stimulated to undergo <shoot multiplication> in 4 and 2, for L. hispanicus and L. albus, respectively. Plantlets obtained from all experiments were easily potted.