Transcriptomic 'portraits' of canine mammary cancer cell lines with various phenotypes

• Authors: M. Krol , K.M. Pawlowski , J. Skierski , P. Turowski , A. Majewska , J. Polanska , M. Ugorski , R.E. Morty , T. Motyl
• Languages of publication: EN

Abstracts

EN

In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, while ABR (active BCR-related) and TMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression of PGP (P glycoprotein), SEMA3B (semaphorin 3B), and STIM1 (stromal interaction molecule 1).

Keywords

EN

CANINE MAMMARY CANCER CELL; DNA MICROARRAY; TRANSCRIPTOMICS

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2010
• Volume: 51
• Issue: 2
• Pages: 169-183

Contributors

author

M. Krol

author

K.M. Pawlowski

author

J. Skierski

author

P. Turowski

author

A. Majewska

author

J. Polanska

author

M. Ugorski

author

R.E. Morty

author

T. Motyl

• Document Type: ARTICLE
• Publication order reference: T. Motyl, Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, Warsaw, Poland