Cytogenetic analysis of an asymmetric potato hybrid

• Authors: M. Srebniak , O. Rasmussen , J. Maluszynska
• Languages of publication: EN

Abstracts

EN

An asymmetric potato hybrid and its parental lines were cytogenetically examined. DAPI (4?-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none of the minichromosomes contains rDNA repeats. CMA (chromomycin A3) band occurred to be a new chromosome marker that identifies potato chromosome No.1. It was possible to detect a deletion in one of four chromosomes No. 1 of the asymmetric potato hybrid. On the basis of these analyses a karyotype of the asymmetric hybrid was constructed.

Keywords

EN

ASYMMETRIC HYBRID; MINICHROMOSOME; POTATO; RDNA; SILVER STAINING

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2002
• Volume: 43
• Issue: 1
• Pages: 19-31

Contributors

author

M. Srebniak

author

O. Rasmussen

author

J. Maluszynska

• Document Type: ARTICLE
• Publication order reference: J. Maluszynska, Department of Plant Anatomy and Cytology, Silesian University, ul. Jagiellonska 28, 40-032 Katowice, Poland