Postmicrosomal protein fractions from short-time-predegenerated rat sciatic nerve

• Authors: D. Gorka , Lewin-Kowalik J. Lewin-Kowalik , J. Lewin-Kowalik , Larysz-Brysz M. Larysz-Brysz , M. Larysz-Brysz , Golka B. Golka , B. Golka , Swiech-Sabuda E. Swiech-Sabuda , E. Swiech-Sabuda , Malecka-Tendera E. Malecka-Tendera , E. Malecka-Tendera
• Languages of publication: EN

Abstracts

EN

The postmicrosomal protein fraction obtained from distal stumps of rat sciatic nerves at 0-6 days following transection were investigated by means of one- and two-dimensional electrophoresis. In all experimental groups, total amount of protein was significantly higher than in the control group. Proteins were resolved into 27 bands after SDS-PAGE. Their molecular weights ranged between 16.2 and 335.4 kDa. Eleven fractions displayed significant quantitative differences. After 2-D-electrophoresis, the pI of the proteins ranged from 4.2 to 7.4. They were resolved to 28 molecular masses from 13.5 kDa to 335.4 kDa. The greatest numbers of fractions (90-109) were observed on the 3rd, 4th, 5th and 6th day after nerve transection. Thus, during first 6 days after transection intensive changes in protein fraction content and composition take place in the distal stump of peripheral nerve. These processes seem to be most prominent on the 4th day after lesion. Results confirm our earlier in vivo findings.

Keywords

EN

DEGENERATION; ELECTROPHORESIS; POSTMICROSOMAL FRACTIONS; PROTEIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2000
• Volume: 60
• Issue: 4
• Pages: 437-445

Contributors

author

D. Gorka

author

Lewin-Kowalik J. Lewin-Kowalik

author

J. Lewin-Kowalik

author

Larysz-Brysz M. Larysz-Brysz

author

M. Larysz-Brysz

author

Golka B. Golka

author

B. Golka

author

Swiech-Sabuda E. Swiech-Sabuda

author

E. Swiech-Sabuda

author

Malecka-Tendera E. Malecka-Tendera

author

E. Malecka-Tendera

• Document Type: ARTICLE
• Publication order reference: D. Gorka, Department of Physiology, Silesian Medical University, 18 Medykow St., 40-762 Katowice, Poland