Ferrous ion induced photon emission as a method to quantify oxidative stress in stored boar spermatozoa
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The aim of the study was to evaluate the effect of semen storage on ferrous ion induced luminescence of boar spermatozoa and to determine the relationship between parameters of this luminescence and lipid peroxidation as measured by malondialdehyde (MDA) contents. Boar semen samples were diluted in Biosolwens extender and stored for 12 days at 15oC. Luminescence andMDA were measured directly after dilution (day 0) and at 6 and 12 days of semen storage. Luminescence was measured at 20oC using a luminometer equipped with a cooled photomultiplier with a spectral response range from 370 to 620 nm. Emission was induced by adding FeSO4 solution (final concentration 0.05mM). MDA content was measured by the HPLC method. The day of storage had a significant effect on some luminescence parameters and MDA content in spermatozoa. A significant correlation was observed between luminescence parameters andMDA concentration. The results of the study confirm that induced luminescence is strictly related to lipid peroxidation in spermatozoa that occur during boar semen storage. Parameters of luminescence treated as a holistic response of cells to oxidati semen preservation.
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Piotr Gogol, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Krakow, Poland