The production of biomass by Yarrowia lipolytica on crude rapeseed oil and waste fatty acids and its characteristics were studied. The biomass obtained in both lipid media had high biological value in the range of 73-78%. The amount of essential aminoacids was in agreement with the FAO standards for fodder yeast, with lysine and phenylalanine which were present in the yeast biomass in higher amounts. The biomass contained 31,3-50,9% of protein and 11,9-28,1% of lipid depending on the yeast strain used. Yarrowia lipolytica ATCC 8661 turned out to be the most suitable strain for the production of SCP on lipid substrates.