A nucleopolyhedrovirus was isolated and characterized for the first time from Leucoma salicis L. (Lepidoptera, Lymantriidae) in Turkey. The virus was observed in populations of L. salicis in G?m??hane. The dimensions of the polyhedrae fell between 2.08'0.31 (1.51-2.64) Fm (N=50). Virions contain 2 to 15 nucleocapsids per virion as seen in cross-section of polyhedrae. The sizes of the viral particles ranged between 250-290 x 32-40 nm. The virus was determined as a Turkish isolate of Leucoma salicis nucleopolyhedrovirus (LesaNPV-TR).