Effect of Pseudomonas aeruginosa crude proteolytic fraction on antibacterial activity of Galleria mellonella Haemolymph

• Authors: M. Andrejko
• Languages of publication: EN

Abstracts

EN

The antibacterial activity of immune haemolymph Galleria mellonella directed against Escherichia coli D31 was destroyed by Pseudomonas aeruginosa crude proteolytic fraction. This was demonstrated by diffusion well assay and acid gel electrophoresis and subsequent bioautography. On the contrary, lysozyme activity appeared to be insensitive to extracellular proteases of P. aeruginosa when activity was determined using the bioautography method. In addition, no change in lysozyme protein level was observed by immunoblotting with specific antibodies directed against G. mellonella lysozyme, which confirmed that lysozyme was not degraded by the crude proteolytic fraction of P. aeruginosa. However, a significant decrease of lysozyme activity in naive and immune haemolymph exposed to the action of P. aeruginosa proteins determined by using diffusion well assay was observed. Mechanisms of the observed inhibition require further studies.

Keywords

EN

ANTIMICROBIAL PEPTIDE; GALLERIA MELLONELLA; LYSOZYME; PROTEASE; PSEUDOMONAS AERUGINOSA

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Journal: Folia Biologica
• Year: 2004
• Volume: 52
• Issue: 1-2
• Pages: 91-96

Contributors

author

M. Andrejko

• Document Type: ARTICLE
• Publication order reference: Mariola Andrejko, Department of Invertebrate Immunology, Maria Curie-Sk?odowska University, Akademicka 19, 20-033 Lublin, Poland