Cytogenetic investigations were carried out on seven species of bisexual weevils from five subfamilies. The following numbers of chromosomes were found in individual species: 2n=22, n_=10+Xyp in Ptochus porcellus Boh. (Otiorhynchinae); 2n=40, n_=19+Xyp in Larinodontes turbinatus Gyll. (Cleoninae); 2n=26, n_=12+Xyp in Curculio pellitus (Boh.) (Curculioninae); 2n=30, n_=14+Xyp in Acalles camelus (F.), Acalles echinatus (Germ.), Acalles hypocrita Boh. (Cryptorhynchinae), and 2n=40, n_=19+Xyp in Cionus hortulanus (Foucr.) (Mecininae). The heterochromosomes of all the examined species form, in the first meiotic metaphase, a parachute bivalent. The chromosome number and sex determining system of six species and two genera are described for the first time.