Disaccharide sucrose plays important role in plants in photoassimilate partitioning, and as a carbon and energy source, it regulates cell metabolism, plant growth and development. Utilization of sucrose in most metabolic pathways is preceding by enzymatic cleavage of sucrose to monosaccharides. This cleavage is catalyzed by invertase and sucrose synthase. Hydrolysis of sucrose to glucose and fructose provides substrates to primary metabolism reactions proceed in plant cell. Because sugars in plants are not only nutrients molecules, but also regulate expression of genes, invertase can control cell division and plant development. Several types of invertases can exist in plant cells, which differ in their biochemical properties and cell localization. The rapid accumulation of information about their physiological function and regulation in planta has revealed the biotechnological potential of these proteins. This review focuses on recent advances in the properties and role of the identified plant invertases in the light of application for plant metabolic engineering.