EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

• Authors: H.-Y. Wang , Y.-M. Wei , Z.-H. Yan , Y.-L. Zheng
• Languages of publication: EN

Abstracts

EN

SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which trinucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.

Keywords

EN

GENETIC DIVERSITY; TRITICUM DURUM

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2007
• Volume: 48
• Issue: 3
• Pages: 35-42

Contributors

author

H.-Y. Wang

author

Y.-M. Wei

author

Z.-H. Yan

author

Y.-L. Zheng

• Document Type: ARTICLE
• Publication order reference: Y.L. Zheng, Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan 625014, China