The activity of chitin deacetylase and glycosidases of the crude enzyme extracts of Mucor rouxii mycelium

• Authors: I. Kolodziejska , A. Wojtasz-Pajak , M. Malesa-Ciecwierz , C. Niecikowska
• Languages of publication: EN

Abstracts

EN

The crude enzyme extract obtained from Mucor rouxii had optimal deacetylasde activity against chitosan acetylated in 39% in the Ph range of 5,2-5.9. Its activity against watger-soluble chitoologosaccharides, containing from 3 to 6 N-acetylglucosamine residues, was the higest in respect to (GlcNAc)s and nil against substrates containing less than 4 N-acetylglucosamine residues.Crystaline chitin was resistant to the deacetylase and to glycosidases present in the extract; amorphous chitin was noticeably more susceptible to these enzymes.Chitosan in the form of suspension was slightly deacetylated and hydrolysed by enzyme extracts.Solubilized chitosan acetylated in 39% was mostly susceptible to enzymatic deacetylation.The deacetylase activity in the extracts was inversely related and the chitonolytic activity was proportional to the deacetylation degree of soluble chitosan.

Keywords

EN

CHITIN; CHITIN DEACETYLASE; CHITINASE; CHITOSAN; CHITOSANASE; GLYCOSIDASE; MUSCOR ROUXII

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1997
• Issue: 2
• Pages: 158-169

Contributors

author

I. Kolodziejska

author

A. Wojtasz-Pajak

author

M. Malesa-Ciecwierz

author

C. Niecikowska

• Document Type: article
• Publication order reference: I.Kolodziejska, PolitechnikaGdanska, Wydzial Chemiczny, Katedra Technologii Utrwalania Zywnosci, Narutowicza 11/12, 80-952 Gdanska, Poland