The activity of chitin deacetylase and glycosidases of the crude enzyme extracts of Mucor rouxii mycelium
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The crude enzyme extract obtained from Mucor rouxii had optimal deacetylasde activity against chitosan acetylated in 39% in the Ph range of 5,2-5.9. Its activity against watger-soluble chitoologosaccharides, containing from 3 to 6 N-acetylglucosamine residues, was the higest in respect to (GlcNAc)s and nil against substrates containing less than 4 N-acetylglucosamine residues.Crystaline chitin was resistant to the deacetylase and to glycosidases present in the extract; amorphous chitin was noticeably more susceptible to these enzymes.Chitosan in the form of suspension was slightly deacetylated and hydrolysed by enzyme extracts.Solubilized chitosan acetylated in 39% was mostly susceptible to enzymatic deacetylation.The deacetylase activity in the extracts was inversely related and the chitonolytic activity was proportional to the deacetylation degree of soluble chitosan.
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I.Kolodziejska, PolitechnikaGdanska, Wydzial Chemiczny, Katedra Technologii Utrwalania Zywnosci, Narutowicza 11/12, 80-952 Gdanska, Poland