The activity of chitin deacetylase and glycosidases of the crude enzyme extracts of Mucor rouxii mycelium

• Authors: I. Kolodziejska , A. Wojtasz-Pajak , M. Malesa-Ciecwierz , C. Niecikowska
• Languages of publication: EN

Abstracts

EN

The crude enzyme extract obtained from Mucor rouxii had optimal deacetylasde activity against chitosan acetylated in 39% in the Ph range of 5,2-5.9. Its activity against watger-soluble chitoologosaccharides, containing from 3 to 6 N-acetylglucosamine residues, was the higest in respect to (GlcNAc)s and nil against substrates containing less than 4 N-acetylglucosamine residues.Crystaline chitin was resistant to the deacetylase and to glycosidases present in the extract; amorphous chitin was noticeably more susceptible to these enzymes.Chitosan in the form of suspension was slightly deacetylated and hydrolysed by enzyme extracts.Solubilized chitosan acetylated in 39% was mostly susceptible to enzymatic deacetylation.The deacetylase activity in the extracts was inversely related and the chitonolytic activity was proportional to the deacetylation degree of soluble chitosan.

Keywords

EN

CHITIN   CHITIN DEACETYLASE   CHITINASE   CHITOSAN   CHITOSANASE   GLYCOSIDASE   MUSCOR ROUXII  

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1997
• Issue: 2
• Pages: 158-169
• Document Type: article
• Publication order reference: I.Kolodziejska, PolitechnikaGdanska, Wydzial Chemiczny, Katedra Technologii Utrwalania Zywnosci, Narutowicza 11/12, 80-952 Gdanska, Poland