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Journal
2001 | 3 | 53-70
Article title

Enzyme-linked immunosorbent assay (ELISA) as a method for detection of specific antibodies in animal sera

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EN
Abstracts
EN
This paper aims to evaluate different forms of the enzyme-linked immunosorbent assays (ELISA) for the detection of virus-specific antibodies and focuses on factors which may influence the diagnostic reliability of such tests. The differences between competitive and non-competitive ELISAs are described, with reference to the influence of the antigen, the conjugated antibody, and the test sample on the test results. Attention is drawn to interference, which may result in false positive or false negative test results. The use of monoclonal antibodies and discriminatory tests is briefly discussed. Diagnostic reliability is described for tests that are used in monitoring or eradication programmes, emphasising the consequences of false negative or false positive results.
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Journal
Year
Issue
3
Pages
53-70
Physical description
References
Document Type
ARTICLE
Publication order reference
M. Gut-Winiarska, Zaklad Wirusologii Molekularnej, Miedzyuczelniany Wydzial Biotechnologii, ul. Kladki 24, 80-822, Gdansk, Poland
YADDA identifier
bwmeta1.element.element-from-psjc-7fe654a8-6c74-342a-bf8e-d3551ea769e0
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