Enhancement of [3H]D-aspartate release during ischemia like conditions in rat hippocampal slices:source of excitatory amino acids

• Authors: B. Zablocka , K. Domanska-Janik
• Languages of publication: EN

Abstracts

EN

Ischemic neuronal injury is supposed to be caused in part by the extracellular accumulation of excitatory amino acids (EAA). Neurotransmitter and metabolic EAA can be released from synaptic vesicles and cytoplasm of neurones and glial cells. In this study the release of the glutamate analogue [3H]D-aspartate (3[H]D-ASP), loaded into 500 mugm slices of rat hippocampus, was investigated. The efflux of the label was measured during anoxic - aglycemic ("ischemic") and normoxic K+ depolarization. To identify the pools from which [3H]D-ASP is released we have estimated its calcium dependence and the effects of inhibitors of: (1) Na+ - dependent transporter of amino acids (100 mugM L-trans-pyrrolidine-2,4-dicarboxylic acid /L-trans-PDC/), (2) sodium channel (1 mugM tetrodotoxin TTX), and (3) anion channel (1mM furosemide). [3H]D-ASP released upon normoxic depolarization was 40% inhibited by TTX,nearly 40% by L-trans-PDC and over 50% by furosemide. The "ischemic" release was in 40% calcium dependent, completely TTX independent and in approximately 50% blocked by furosemide treatment. Our data suggest that EAA accumulated in the synaptic cleft during ischemia are mainly released from the cytosolic compartment by mechanisms wich are connected with the ischemic increase of extracellular potassium concentration.

Keywords

EN

D-ASPARTATE; EAA COMPARTMENTS; EXCITATORY AMINO ACID; HIPPOCAMPAL SLICE; ISCHAEMIA IN VITRO

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 1996
• Volume: 56
• Issue: 1
• Pages: 63-70

Contributors

author

B. Zablocka

author

K. Domanska-Janik

• Document Type: proceeding
• Publication order reference: B.Zablocka