Classic techniques for improvement of industrial yeast strains: Part III ? A method for enucleation of Saccharomyces sp.
Languages of publication
The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCl2 and ATP (10 muM) in the enucleation medium prevented the formation of fragile products or aggregation. Cytoplasts could be stored for at least 1 day without visible deterioration.
Publication order reference
A.T. Salek, Milan-Science, Institut of Microbiology, Dr. Ernst-Derra Str. 4, 94036 Passau, Germany