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Journal
2002 | 1 | 187-194
Article title

Classic techniques for improvement of industrial yeast strains: Part III ? A method for enucleation of Saccharomyces sp.

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EN
Abstracts
EN
The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCl2 and ATP (10 muM) in the enucleation medium prevented the formation of fragile products or aggregation. Cytoplasts could be stored for at least 1 day without visible deterioration.
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Journal
Year
Issue
1
Pages
187-194
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ARTICLE
Publication order reference
A.T. Salek, Milan-Science, Institut of Microbiology, Dr. Ernst-Derra Str. 4, 94036 Passau, Germany
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YADDA identifier
bwmeta1.element.element-from-psjc-7d6e9615-fb85-38a8-a722-b6b8d82c6dfc
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