PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2007 | 3 | 123-140
Article title

Optimization of transduction of chosen cell lines using adenoviral vetors of the first generation

Title variants
Languages of publication
PL
Abstracts
EN
Efficacy of adenoviral vectors (AdV) was checked by transduction of six different cell lines (COS-7, HUVEC, HMEC-1, NIH 3T3, HaCaT, and B16(F10)) with the vectors containing reporter gene beta-galactosidase (Adbeta-gal). We optimised the adsorption time and dose of Ad beta-gal. The transduction with efficacy of up to 100% was obtained for the doses 10-100 IU/cell. In order to examine the effect of transduction procedure on biology of the cells, we measured the production of major proinflammatory cytokines. In several cell lines (HMEC-1, HUVEC, HaCaT), we found the induction of IL-6 synthesis and decrease in the cell viability, particularly in the case of the highest Adbeta-gal dose. However, the treatment with adenoviral vectors did not induce TNF generation and did not modify proliferation of cells.
Publisher

Journal
Year
Issue
3
Pages
123-140
Physical description
Contributors
author
author
author
References
Document Type
ARTICLE
Publication order reference
Alicja Jozkowicz, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-7a48e8cd-1eeb-3fbe-8b6d-c7dd7d642508
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.