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Journal
2008 | 56 | 1-2 | 31-35
Article title

Analyses of the structure of NORs in two species of south American Sorubiminae fishes (Siluriformes) by means of several cytogenetic techniques

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Languages of publication
EN
Abstracts
EN
Several cytogenetic techniques (AgNOR, C- G- and RE bandings, DAPI, CMA3 and FISH) were applied in order to analyze the structure and variability of NORs in the fish species Steindachneridion melanodermatum and S. scripta. Ag-NORs were observed on the short arm of the first acrocentric chromosome pair, coincidentally with a strong C-positive band on a large secondary constriction. In addition, NORs showed bright fluorescent signals when stained with CMA3 and treated for FISH with rDNA 18S. However, they showed negative coloration after G- and restriction enzyme banding and DAPI staining. The results evidence a substantial size polymorphism in these regions. The NOR bearing chromosomes in both species may be considered homologues because they maintain conserved characteristics, such as being interspersed with a GC-rich heterochromatin and possessing target sequences for AluI, BamHI and EcoRI.
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Journal
Year
Volume
56
Issue
1-2
Pages
31-35
Physical description
Contributors
author
author
author
References
Document Type
ARTICLE
Publication order reference
Ana Claudia Swarca, Universidade Estadual de Londrina, Centro de Ciencias Biologicas, Caixa Postal 6001, 86051-970 Londrina, Parana, Brasil
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YADDA identifier
bwmeta1.element.element-from-psjc-77e0bc3b-48b4-3cb7-9c75-d487722ab0aa
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