Flow cytometry in mammalian sperm sexing and quality measurement
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Flow cytometry is a powerful technique for measurements of many structural and functional cell parameters. In sperm analyses it offers several advantages over the formerly used methods: high accuracy, possibility of measuring two or even three parameters simultaneously and analysis of a high number of cells per second. Moreover, at present it is the only technique which allows sperm sorting of X- and Y-fractions with high purity. The paper is a review of the flow cytometry applications in sperm sexing and measurements of quality parameters: membrane integrity, acrosome status, mitochondrial and esterase activity and sperm chromatin structure.
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M. Bochenek, Zaklad Fizjologii Rozrodu Zwierzat, Instytut Zootechniki, 32-083 Balice k. Krakowa, Poland