Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose

• Authors: E. Katzenellenbogen , N.A. Kocharova , A. Korzeniowska-Kowal , A. Gamian , M. Bogulska , B. Szostko , A.S. Shashkov , Y.A. Knirel
• Languages of publication: EN

Abstracts

EN

Introduction: Hafnia alvei is the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. So far, 29 different O-polysaccharide (OPS, O-antigen) structures in Hafnia LPSs have been established and for some of them the molecular basis of the serological activity has been elucidated. Materials and Methods: OPS from H. alvei strain PCM 1219 was obtained by mild acid hydrolysis of the LPS followed by gel permeation chromatography of carbohydrate material on Sephadex G-50 column. The polysaccharide structure was determined using chemical methods as well as 13C NMR and 1H NMR spectroscopy. For serological studies, SDS-PAGE, immunoblotting, and passive hemagglutination tests were used. Results: The serological studies revealed a cross-reactivity of the LPSs of H. alvei PCM 1219 and a group of H. alvei strains with an O-antigen containing D-glucose 1-phosphate and [(R)-3-hydroxybutyramido]-D-glucose. The following structure of the OPS was established: 2)-alpha-d-Glcp-(1-PO4-6)-alpha-d-GlcpNAcyl-(14)-alpha-d-GalpNAc-(13)-beta-d-GalpNAc-(1- > 3---- OAc6<-1 alpga-d-Glcp where Acyl stands for (R)-3-hydroxybutyryl and the degree of O-acetylation is 70%. The structure of the core oligosaccharide was found to be typical of the genus Hafnia. Conclusions: Based on the OPS structure and serological results it was concluded that H. alvei strain PCM 1219 should be classified in the same serogroup as the H. alvei type strain ATCC 13337 and five other strains containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose in their O-antigens.

Keywords

EN

BACTERIAL POLYSACCHARIDE; ENTEROBACTERIA; HAFNIA ALVEI; LIPOPOLYSACCHARIDE; O-ANTIGEN

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2008
• Volume: 56
• Issue: 5
• Pages: 347-352

Contributors

author

E. Katzenellenbogen

author

N.A. Kocharova

author

A. Korzeniowska-Kowal

author

A. Gamian

author

M. Bogulska

author

B. Szostko

author

A.S. Shashkov

author

Y.A. Knirel

• Document Type: ARTICLE
• Publication order reference: Ewa Katzenellenbogen, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroc?aw, Poland