Presence of env-like sequences in Quercus suber retrotransposons

• Authors: M. Carvalho , T. Ribeiro , W. Viegas , L. Morais-Cec?lio , M. Rocheta
• Languages of publication: EN

Abstracts

EN

The main difference between LTR retrotransposons and retroviruses is the presence of the envelope (env) gene in the latter, downstream of the pol gene. The env gene is involved in their infectious capacity. Here we report the presence of env-like sequences in the genome of Quercus suber (cork oak), one of the most economically important Portuguese species. These gene sequences were isolated through DNA amplification between RNaseH conserved motifs and 3' LTR, based on the structure of copia retrotransposons. Phylogenetic analysis revealed that almost all the clones isolated are clustered with Cyclops-2, a Ty3-gypsy element identified in Pisum sativum, except one clustered with gypsy and copia retroelements found in different species. This suggests the existence of a potential ancestral sequence of the env gene, prior to the separation of Ty3-gypsy and Ty1-copia retrotransposons. Additionally, the isolated env-like sequences showed 26?39% of homology with env-like sequences characterized in viruses. The origin of env-like sequences in retrotransposons from host plant taxa is discussed.

Keywords

EN

COPIA RETROELEMENT; CORK OAK; RETROTRANSPOSON; RETROVIRUS

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2010
• Volume: 51
• Issue: 4
• Pages: 461-467

Contributors

author

M. Carvalho

author

T. Ribeiro

author

W. Viegas

author

L. Morais-Cec?lio

author

M. Rocheta

• Document Type: ARTICLE
• Publication order reference: M. Rocheta, Instituto Superior de Agronomia, Universidade T?cnica de Lisboa, Centro de Botanica Aplicada r Agricultura, Tapada da Ajuda, 1349-017 Lisboa, Portugal