The induction of haploids of rye (Secale cereale L.) by the anther culture
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Haploid plants are often used for genome mapping, to induce mutations and also for the production of homozygous plants. They are also needed in heterosis breeding. Androgenesis is one of the methods of their obtainment. The study focused on the development of induction haploid plants and double haploid lines of rye. In the experiment, seven varieties of rye were used: Amilo, Dankowskie Nowe, Dankowskie Z?ote, Kier, Motto, Walet, Warko. The seedlings were vernalised at natural conditions or in the refrigerator. Plants were grown in the greenhouse. Spikes with pollen at the uninucleate stage were harvested and stored at 4 C in the dark for 48 hours. The influence of the varieties and the medium used on the androgenesis was tested. Modified N6 and potato medium were used. Anthers were cultivated in the dark for 4 weeks at 28C. Very low incidence of callus-producing plants (0,0-0,3%) and of any regenerated plants was observed.
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Z. Broda, Katedra Genetyki i Hodowli Roslin, Akademia Rolnicza im. Augusta Cieszkowskiego, ul. Wojska Polskiego 71C, 60-625 Poznan, Poland