Effects of DAGO on the rodent hippocampal evoked potentials using different perfusion solutions

• Authors: Martin S. San_ , L. Menendez , M. Gutierrez , A. Hidalgo , A. Baamonde
• Languages of publication: EN

Abstracts

EN

Opioid receptor agonists exert excitatory effects in the hippocampus by inhibiting GABA release. We report that the mu-opioid agonist, DAGO, increases the amplitude of the population spikes (PS) measured in the stratum pyramidale of the CA1 cell layer in mouse and rat hippocampal slices perfused with an artificial cerebrospinal fluid (ACSF), but not when perfused in Krebs solution. The GABA A agonist, 3-APS, induces inhibitory responses when perfused in either ACSF or Krebs. Also, the field excitatory postsynaptic potentials (EPSP) measured on stratum radiatum do not differ when the slice is perfused with either ACSF or Krebs. The increase in the amplitude of the PS induced by DAGO is not obtained when perfused in a modified ACSF whose concentration of MgSO4 was lowered to its concentration in the Krebs solution (from 2.4 mM to 1.2 mM). Thus, changes in the concentration of MgSO4 seem to be responsible for the different responses induced by DAGO.

Keywords

EN

GABA; HIPPOCAMPAL SLICE; HIPPOCAMPUS; MOUSE; RAT

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2000
• Volume: 60
• Issue: 2
• Pages: 187-193

Contributors

author

Martin S. San_

author

L. Menendez

author

M. Gutierrez

author

A. Hidalgo

author

A. Baamonde

• Document Type: article
• Publication order reference: A. Baamonde, Laboratorio de Farmacologia, Departamento de Medicina, Facultad de Medicina, C/ Julian Claveria s/n, 33006 Oviedo, Spain, Email: [e-mail]