Unidirectional orientation of twelve expressed tagged sites within 40 kb of human chromosomal region 22q13.1
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. The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.
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N. Blin, Institute of Anthropology and Human Genetics, Wilhelmstr. 27, 72074 T?bingen, Germany, E-mail: firstname.lastname@example.org.