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2001 | 49 | 3 | 253-259
Article title

In vitro culture of human epithelial cells on a modified xenogenic collagen support

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EN
Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.
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E. Lesiak-Cyganowska, Department of Transplantology and Central Tissue Bank, Center of Biostructure Research, Warsaw Medical University, Chalubinskiego 5, 02-004 Warsaw, Poland
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bwmeta1.element.element-from-psjc-6d7fc913-3b77-37c2-8e88-9e657a90cc64
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