MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro

• Authors: S. Kabadere , P. Oztopcu , S. Korkmaz , K. Erol , R. Uyar
• Languages of publication: EN

Abstracts

EN

In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol- -2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 mM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2004
• Volume: 64
• Issue: 4
• Pages: 461-466

Contributors

author

S. Kabadere

author

P. Oztopcu

author

S. Korkmaz

author

K. Erol

author

R. Uyar

• Document Type: ARTICLE
• Publication order reference: Ruhi Uyar, Department of Physiology, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey