Germin genes expression induced by stress factors in transgenic tobacco plants (Nicotiana tabacum var. Xanthi)

• Authors: J. Nowakowska
• Languages of publication: PL

Abstracts

EN

Wheat germin, a glycoprotein mainly localized in the cell wall of monocots, dicots and pine (Pinus caribaea Morelet), is a specific marker of the onset of growth in germinating seeds. There are two genes gf-2.8 and gf-3.8 of germin, isolated from wheat (Triticum aestivum). Because of their structure and biochemistry, both of the germin's isoforms have been now assimilated to the oxalate-oxidase enzyme (E.C. 1.2.3.4). Several constructs of the gf-2.8 and gf-3.8 genes promoters deletion were fused with uidA gene and tested for the GUS activity in the transgenic tobacco plants. The aim of this work was to evaluate whether some environmental factors induce the activity of germin. Hormonal treatment (auxins 2,4-D, NAA, gibberellin GA3), salt treatment, salicylic acid, heavy metals (Mn, Fe, Co, Ni, Cu, Zn, Cd, Hg, As) and A1 induced high GUS activity in root apex, cotyledons and hypocotyls in transgenic seedlings and in leaves of the tobacco plants transformed with the entire and one partially deleted construct of the gf-2.8 gene. Neither cold nor heat shock, enhanced significant GUS activity. The gf-3.8 - GUS constructs of germin did not give a positive response to any factors mentioned above. Thus, only the gf-2.8 protein seems to be regulated by some factors. The gf-2.8 oxalate-oxidase activity could be then involved in general stress-induced signalling in higher plants

Keywords

EN

AUXIN; COLD; GERMIN; GIBBERELLIC ACID; HEAT SHOCK; HEAVY METAL; OXALATE OXIDASE; SALICYLIC ACID

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2001
• Issue: 2
• Pages: 152-164

Contributors

author

J. Nowakowska

• Document Type: ARTICLE
• Publication order reference: J. Nowakowska, Zaklad Genetyki i Fizjologii Drzew Lesnych, Instytut Badawczy Lesnictwa, ul. Bitwy Warszawskiej 1920 roku nr 3, 00-973 Warszawa, Poland, e-mail: [e-mail]