The method of chitosan separation from fungus cell walls was presented by White, Farina, Fulton in 1979. Since that time many other investigators worked on that subject. None of them succeeded in developing a technology that would be worth-while as faras the economical aspect of the matter is concerned. The yield of chitosan from fungal mass or from cultivation medium depends on several factors like: strain of fungi used, cultivation method (submerged cultures or solid-state cultures), cultivation parameters (pH, temperature, time of cultivation). The aim of this paper was to compare the literature and own data presenting the influence of culture conditions on chitosan yields.