Patterned growth and differentiation of human cord blood- derived neural stem cells on bio-functionalized surfaces

• Authors: L. Buzanska , A. Ruiz , M. Zychowicz , H. Rauscher , L. Ceriotti , F. Rossi , P. Colpo , K. Domanska-Janik , S. Coecke
• Languages of publication: EN

Abstracts

EN

Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells. development in the large scale multiparameter biotests.

Keywords

EN

CORD BLOOD; MICROPATTERNING; NEURAL STEM CELL

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2009
• Volume: 69
• Issue: 1
• Pages: 24-36

Contributors

author

L. Buzanska

author

A. Ruiz

author

M. Zychowicz

author

H. Rauscher

author

L. Ceriotti

author

F. Rossi

author

P. Colpo

author

K. Domanska-Janik

author

S. Coecke

• Document Type: ARTICLE
• Publication order reference: Leonora Buzanska, NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland