Chromosome pairing in triploid intergeneric hybrids of Festuca pratensis with Lolium multiflorum, revealed by GISH

• Authors: A. Kosmala , E. Zwierzykowska , Z. Zwierzykowski
• Languages of publication: EN

Abstracts

EN

Genomic in situ hybridisation (GISH) was used to reveal chromosome pairing in two partly fertile, triploid (2n = 3x = 21) hybrids obtained by crossing the diploid (2n = 2x = 14) Festuca pratensis Huds. (designated FpFp), used as a female parent, with the autotetraploid (2n = 4x = 28) Lolium multiflorum Lam. (designated LmLmLmLm), used as a male parent. The pattern of chromosome pairing calculated on the basis of the mean values of chromosome configurations identified in all 100 PMCs analysed, was: 0.71I Lm + 2.24I Fp + 2.18II Lm/Lm + 0.54II Lm/Fp + 4.18III Lm/Lm/Fp. A relatively high number of Lm/Lm bivalents and Fp univalents, and a low number of Lm/Fp bivalents and Lm univalents indicated that the pairing was preferential between L. multiflorum chromosomes. Other observations regarding chromosome pairing within the Lm/Lm/Fp trivalents also confirmed this preferential pairing in the analysed triploids, as the Fp chromosome was not randomly located in the chain- and frying-pan-shaped trivalents. The similarities and differences in chromosome pairing at metaphase I and the level of preferential pairing between Lolium chromosomes in the different triploid Lolium-Festuca hybrids are discussed.

Keywords

EN

CHROMOSOME PAIRING; FESTUCA PRATENSIS; INTERGENERIC HYBRID; LOLIUM MULTIFLORUM; TRIPLOID

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 2006
• Volume: 47
• Issue: 3
• Pages: 215-220

Contributors

author

A. Kosmala

author

E. Zwierzykowska

author

Z. Zwierzykowski

• Document Type: ARTICLE
• Publication order reference: A. Kosmala, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60?479 Poznan, Poland