The use of amelogenin gene polymorphism in PCR embryo sexing in bovine IVF embryos

• Authors: D. Lechniak , I.R. Cumming
• Languages of publication: EN

Abstracts

EN

The present study describes a rapid, simple method of bovine IVF embryo sexing by use of PCR technique. A pair of primers corresponding to the bovine amelogenin sequence has been used. The Rapid Cycler (Idaho Technology, USA) used in the current experiment enabled the PCR programme consisting of 55 cycles to be completed in less than 40 minutes. Therefore the total sexing procedure could be performed in less than 90 minutes. The described method succeded in case of 85% analysed embryos.

Keywords

EN

AMELOGENIN; BOVINE EMBRYO; PCR

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 1
• Pages: 45-49

Contributors

author

D. Lechniak

author

I.R. Cumming

• Document Type: article
• Publication order reference: D.Lechniak, Department of Genetics and Animal Breeding, Agricultural University, Wolynska 33, 60-637 Poznan, Poland