Cucumber transformation methods - the review

• Authors: Z. Yin , G. Bartoszewski , M. Szwacka , S. Malepszy
• Languages of publication: EN

Abstracts

EN

Several aspects of cucumber transformation including the ways of transgene introduction, factors influencing the transformation efficiency and the fate of the introduced genes were reviewed. Various transgenes have been introduced into the cucumber genome mostly via the Agrobacterium-mediated transformation. The frequency of Agrobacterium-mediated transformation ranged from 0.8 to 10% and was influenced by the selection agent, the regeneration efficiency, activation of vir genes expression, the explant size, bacteria cell density, the length of exposure and the co-cultivation period. The transgenes were integrated mostly as single copy in the Agrobacterium-mediated transformation and as multiple copies in direct transformation. Variable levels of the transgene expression were observed. The transmission of the transgenes as well as the transgenic phenotype follow the Mendelian, and rarely non-Mendelian, ratio. The production of marker-free transgenic cucumber and use of an alternative transformation method are recommended.

Keywords

EN

AGROBACTERIUM; CUCUMIS SATIVUS; MARKER; TRANSFORMATION

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2005
• Issue: 1
• Pages: 95-113

Contributors

author

Z. Yin

author

G. Bartoszewski

author

M. Szwacka

author

S. Malepszy

• Document Type: REVIEW
• Publication order reference: Stefan Malepszy, Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland