Interaction of Antisense oligonucleotides with DNA and proteins studied by fluorescence polarization spectroscopy

• Authors: A. Murakami , M. Nakuara , H. Uemastu , N. Fujimora , R. Iwase , T. Yamaoka
• Languages of publication: EN

Abstracts

EN

In order to evaluate the antisense effects in biological systems, the interaction of antisense molecules with biological substances such as DNAs and human serum proteins was studied by fluorescence depolarization spectroscopy.When an antisense oligonucleotide anchoring fluorescence (F-AS) was mixed with DNAs or proteins, the anisotropy value changed, indicating that antisense oligonucleotides appreciably interact with DNAs and serum proteins, and that such interaction may play important roles on antisense gene regulation.

Keywords

EN

ANTISENSE OLIGONUCLEOTIDE; FLUORESCENCE DEPOLARIZATION SPECTROSCOPY

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1996
• Issue: 4
• Pages: 153-166

Contributors

author

A. Murakami

author

M. Nakuara

author

H. Uemastu

author

N. Fujimora

author

R. Iwase

author

T. Yamaoka

• Document Type: article
• Publication order reference: Akira Murakami, Dept. of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan