Characteristics of SAMPL markers and their utilization in plant genomes researches
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In the last 20 years, several marker systems based on the microsatellite sequences have been elaborated. One of them is SAMPL (Selective Amplification of Microsatellite Polymorphic Loci), which gives a possibillity to detect genetic polymorphisms resulting not only from mutations in restriction sites, but also from different number of microsatellite motif repeats. That makes it a valuable tool for a genetic diversity studies even among closely related forms. Moreover, thanks to the abillity of simultaneous analysis of many segregating loci in one gel line, SAMPL markers are very useful to construct new and improve density of already existing genetic maps.
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Aneta Hromada, Katedra Genetyki, Hodowli i Biotechnologii Roslin, Szkola Glowna Gospodarstwa Wiejskiego, ul. Nowoursynowska 159, 02-776 Warszawa, Poland